selenOtest ELISA

Diagnostik

selenOtest ELISA

Ein kolorimetrischer Enzym-gekoppelter Immuntest für die quantitative Bestimmung von humanem Selenoprotein P (SELENOP) im Serum 

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Produktspezifikationen

Produkt-Name selenOtest ELISA
Synonyme Selenoprotein P ELISA, SELENOP ELISA,
SEPP1 ELISA
Produkt-Code STE
Verwendungszweck Der selenOtest ELISA wurde für die quantitative Messung von menschlichem Selenoprotein P (SELENOP, SeP, SELP, SEPP1) Konzentrationen in verdünnten Serumproben entwickelt und validiert.
Assay-Format 96 well Mikrotiterplatte
Nachweismethode kolorimetrisch (450 nm)
Reaktivität Human
Probenvolumen* 5 – 10 µL
Probenart Serum
Assay Zeit 5 – 6 Stunden
Assay Sensitivität* Ca. 10 µg/L (ungefähr 400-fach unter dem Serumgehalt  eines gesunden Menschens)
Detektionsbereich* Ca. 5 – 800 µg/L
Quantifizierungsbereich* Ca. 10 – 400 µg/L (entspricht 0.4 – 12 mg/L in einer unverdünnten Serumprobe)
Lagerungsbedingungen gekühlt bei 2 – 8°C
Haltbarkeit 6 Monate
Restriktionen Nur für Forschungszwecke.

* Lot spezifische Angaben

Dokumente zum Download:

Lot STE.18001:

Arbeitsanleitung.pdf

Produktspezifikation.pdf

Qualitätszertifikat.pdf

EG-Konformitätserklärung.pdf

Archiv:

Lot STE.16001

Referenzen:

Die nachstehenden Veröffentlichungen verwenden den selenOtest ELISA, einen Sandwich ELISA zur Quantifizierung von  Selenoprotein P (SELENOP ):

2019

Letter by Schomburg and Melander Regarding Article, „Selenoprotein P Promotes the Development of Pulmonary Arterial Hypertension: A Possible Novel Therapeutic Target“.
Schomburg L, Melander O.
Circulation. 2019 Jan 29;139(5):722-723. doi: 10.1161/CIRCULATIONAHA.118.035782.

Selenoprotein P in Patients with Nonalcoholic Fatty Liver Disease.
Polyzos SA, Kountouras J, Mavrouli M, Katsinelos P, Doulberis M, Gavana E, Duntas L.
Exp Clin Endocrinol Diabetes. 2019 Jan 9. doi: 10.1055/a-0811-9136.

Relation of selenium status to neuro-regeneration after traumatic spinal cord injury.
Heller RA, Seelig J, Bock T, Haubruck P, Grützner PA, Schomburg L, Moghaddam A, Biglari B.
J Trace Elem Med Biol. 2019 Jan;51:141-149. doi: 10.1016/j.jtemb.2018.10.006.

2018

Expression of Selenoprotein Genes and Association with Selenium Status in Colorectal Adenoma and Colorectal Cancer.
Hughes DJ, Kunická T, Schomburg L, Liška V, Swan N, Souček P.
Nutrients. 2018 Nov 21;10(11). pii: E1812. doi: 10.3390/nu10111812.

Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits.
Saito Y, Misu H, Takayama H, Takashima SI, Usui S, Takamura M, Kaneko S, Takamura T, Noguchi N.
Biol Pharm Bull. 2018;41(5):828-832. doi: 10.1248/bpb.b18-00046.

Cow Milk Consumption Increases Iodine Status in Women of Childbearing Age in a Randomized Controlled Trial.
O’Kane SM, Pourshahidi LK, Mulhern MS, Strain JJ, Mackle EM, Koca D, Schomburg L, Hill S, O’Reilly J, Kmiotek D, Deitrich C, Bath SC, Yeates AJ.
J Nutr. 2018 Mar 1;148(3):401-408. doi: 10.1093/jn/nxx043.

2017

Double-Blind, Placebo-Controlled, Randomized Trial of Selenium in Graves Hyperthyroidism.
Kahaly GJ, Riedl M, König J, Diana T, Schomburg L.
J Clin Endocrinol Metab. 2017 Nov 1;102(11):4333-4341. doi: 10.1210/jc.2017-01736.

Aminoglycoside-driven biosynthesis of selenium-deficient Selenoprotein P.
Renko K, Martitz J, Hybsier S, Heynisch B, Voss L, Everley RA, Gygi SP, Stoedter M, Wisniewska M, Köhrle J, Gladyshev VN, Schomburg L.
Sci Rep. 2017 Jun 29;7(1):4391. doi: 10.1038/s41598-017-04586-9.

Sex-specific and inter-individual differences in biomarkers of selenium status identified by a calibrated ELISA for selenoprotein P.
Hybsier S, Schulz T, Wu Z, Demuth I, Minich WB, Renko K, Rijntjes E, Köhrle J, Strasburger CJ, Steinhagen-Thiessen E, Schomburg L.
Redox Biol. 2017 Apr;11:403-414. doi: 10.1016/j.redox.2016.12.025.

2016

Prediagnostic selenium status and hepatobiliary cancer risk in the European Prospective Investigation into Cancer and Nutrition cohort.
Hughes DJ, Duarte-Salles T, Hybsier S, Trichopoulou A, Stepien M, Aleksandrova K, Overvad K, Tjønneland A, Olsen A, Affret A, Fagherazzi G, Boutron-Ruault MC, Katzke V, Kaaks R, Boeing H, Bamia C, Lagiou P, Peppa E, Palli D, Krogh V, Panico S, Tumino R, Sacerdote C, Bueno-de-Mesquita HB, Peeters PH, Engeset D, Weiderpass E, Lasheras C, Agudo A, Sánchez MJ, Navarro C, Ardanaz E, Dorronsoro M, Hemmingsson O, Wareham NJ, Khaw KT, Bradbury KE, Cross AJ, Gunter M, Riboli E, Romieu I, Schomburg L, Jenab M.
Am J Clin Nutr. 2016 Aug;104(2):406-14. doi: 10.3945/ajcn.116.131672.

Selenium status in neonates with connatal infection.
Wiehe L, Cremer M, Wisniewska M, Becker NP, Rijntjes E, Martitz J, Hybsier S, Renko K, Bührer C, Schomburg L.
Br J Nutr. 2016 Aug;116(3):504-13. doi: 10.1017/S0007114516002208.

Selenium Status Is Positively Associated with Bone Mineral Density in Healthy Aging European Men.
Beukhof CM, Medici M, van den Beld AW, Hollenbach B, Hoeg A, Visser WE, de Herder WW, Visser TJ, Schomburg L, Peeters RP.
PLoS One. 2016 Apr 7;11(4):e0152748. doi: 10.1371/journal.pone.0152748.

Lack of Association between Selenium Status and Disease Severity and Activity in Patients with Graves‘ Ophthalmopathy.
Dehina N, Hofmann PJ, Behrends T, Eckstein A, Schomburg L.
Eur Thyroid J. 2016 Mar;5(1):57-64. doi: 10.1159/000442440.

2015

Selenium status is associated with colorectal cancer risk in the European prospective investigation of cancer and nutrition cohort.
Hughes DJ, Fedirko V, Jenab M, Schomburg L, Méplan C, Freisling H, Bueno-de-Mesquita HB, Hybsier S, Becker NP, Czuban M, Tjønneland A, Outzen M, Boutron-Ruault MC, Racine A, Bastide N, Kühn T, Kaaks R, Trichopoulos D, Trichopoulou A, Lagiou P, Panico S, Peeters PH, Weiderpass E, Skeie G, Dagrun E, Chirlaque MD, Sánchez MJ, Ardanaz E, Ljuslinder I, Wennberg M, Bradbury KE, Vineis P, Naccarati A, Palli D, Boeing H, Overvad K, Dorronsoro M, Jakszyn P, Cross AJ, Quirós JR, Stepien M, Kong SY, Duarte-Salles T, Riboli E, Hesketh JE.
Int J Cancer. 2015 Mar 1;136(5):1149-61. doi: 10.1002/ijc.29071.

Chronic Kidney Disease Distinctly Affects Relationship Between Selenoprotein P Status and Serum Thyroid Hormone Parameters.
Reinhardt W, Dolff S, Benson S, Broecker-Preuß M, Behrendt S, Hög A, Führer D, Schomburg L, Köhrle J.
Thyroid. 2015 Oct;25(10):1091-6. doi: 10.1089/thy.2015.0097.

Regulation of Selenocysteine Content of Human Selenoprotein P by Dietary Selenium and Insertion of Cysteine in Place of Selenocysteine.
Turanov AA, Everley RA, Hybsier S, Renko K, Schomburg L, Gygi SP, Hatfield DL, Gladyshev VN.
PLoS One. 2015 Oct 9;10(10):e0140353. doi: 10.1371/journal.pone.0140353.

Association between maternal micronutrient status, oxidative stress, and common genetic variants in antioxidant enzymes at 15 weeks׳ gestation in nulliparous women who subsequently develop preeclampsia.
Mistry HD, Gill CA, Kurlak LO, Seed PT, Hesketh JE, Méplan C, Schomburg L, Chappell LC, Morgan L, Poston L; SCOPE Consortium.
Free Radic Biol Med. 2015 Jan;78:147-55. doi: 10.1016/j.freeradbiomed.2014.10.580.

Gene-specific regulation of hepatic selenoprotein expression by interleukin-6.
Martitz J, Becker NP, Renko K, Stoedter M, Hybsier S, Schomburg L.
Metallomics. 2015 Nov;7(11):1515-21. doi: 10.1039/c5mt00211g.

2014

Selenoprotein P is the essential selenium transporter for bones.
Pietschmann N, Rijntjes E, Hoeg A, Stoedter M, Schweizer U, Seemann P, Schomburg L.
Metallomics. 2014 May;6(5):1043-9. doi: 10.1039/c4mt00003j.

Selenite supplementation in euthyroid subjects with thyroid peroxidase antibodies.
Eskes SA, Endert E, Fliers E, Birnie E, Hollenbach B, Schomburg L, Köhrle J, Wiersinga WM.
Clin Endocrinol (Oxf). 2014 Mar;80(3):444-51. doi: 10.1111/cen.12284.

Selenoprotein P in seminal fluid is a novel biomarker of sperm quality.
Michaelis M, Gralla O, Behrends T, Scharpf M, Endermann T, Rijntjes E, Pietschmann N, Hollenbach B, Schomburg L.
Biochem Biophys Res Commun. 2014 Jan 17;443(3):905-10. doi: 10.1016/j.bbrc.2013.12.067.

Hypoxia reduces and redirects selenoprotein biosynthesis.
Becker NP, Martitz J, Renko K, Stoedter M, Hybsier S, Cramer T, Schomburg L.Metallomics. 2014 May;6(5):1079-86. doi: 10.1039/c4mt00004h.

2012

Differential responses to selenomethionine supplementation by sex and genotype in healthy adults.
Combs GF Jr, Jackson MI, Watts JC, Johnson LK, Zeng H, Idso J, Schomburg L, Hoeg A, Hoefig CS, Chiang EC, Waters DJ, Davis CD, Milner JA.
Br J Nutr. 2012 May;107(10):1514-25. doi: 10.1017/S0007114511004715.

Selenoprotein P status correlates to cancer-specific mortality in renal cancer patients.
Meyer HA, Endermann T, Stephan C, Stoedter M, Behrends T, Wolff I, Jung K, Schomburg L.
PLoS One. 2012;7(10):e46644. doi: 10.1371/journal.pone.0046644.

Bone turnover and bone mineral density are independently related to selenium status in healthy euthyroid postmenopausal women.
Hoeg A, Gogakos A, Murphy E, Mueller S, Köhrle J, Reid DM, Glüer CC, Felsenberg D, Roux C, Eastell R, Schomburg L, Williams GR.
J Clin Endocrinol Metab. 2012 Nov;97(11):4061-70. doi: 10.1210/jc.2012-2121.

2011

Comparison of different selenocompounds with respect to nutritional value vs. toxicity using liver cells in culture.
Hoefig CS, Renko K, Köhrle J, Birringer M, Schomburg L.
J Nutr Biochem. 2011 Oct;22(10):945-55. doi: 10.1016/j.jnutbio.2010.08.006.

Determinants of selenium status in healthy adults.
Combs GF Jr, Watts JC, Jackson MI, Johnson LK, Zeng H, Scheett AJ, Uthus EO, Schomburg L, Hoeg A, Hoefig CS, Davis CD, Milner JA.
Nutr J. 2011 Jul 18;10:75. doi: 10.1186/1475-2891-10-75.

2010

Selenoprotein-related disease in a young girl caused by nonsense mutations in the SBP2 gene.
Azevedo MF, Barra GB, Naves LA, Ribeiro Velasco LF, Godoy Garcia Castro P, de Castro LC, Amato AA, Miniard A, Driscoll D, Schomburg L, de Assis Rocha Neves F.
J Clin Endocrinol Metab. 2010 Aug;95(8):4066-71. doi: 10.1210/jc.2009-2611.

The choice of biomarkers determines the selenium status in young German vegans and vegetarians.
Hoeflich J, Hollenbach B, Behrends T, Hoeg A, Stosnach H, Schomburg L.
Br J Nutr. 2010 Dec;104(11):1601-4. doi: 10.1017/S0007114510002618.

Effects of selenium status and polymorphisms in selenoprotein genes on prostate cancer risk in a prospective study of European men.
Steinbrecher A, Méplan C, Hesketh J, Schomburg L, Endermann T, Jansen E, Akesson B, Rohrmann S, Linseisen J.
Cancer Epidemiol Biomarkers Prev. 2010 Nov;19(11):2958-68. doi: 10.1158/1055-9965.EPI-10-0364.

2009

Reduced serum selenoprotein P concentrations in German prostate cancer patients.
Meyer HA, Hollenbach B, Stephan C, Endermann T, Morgenthaler NG, Cammann H, Köhrle J, Jung K, Schomburg L.
Cancer Epidemiol Biomarkers Prev. 2009 Sep;18(9):2386-90. doi: 10.1158/1055-9965.EPI-09-0262.

Serum selenium and selenoprotein P status in adult Danes – 8-year followup.
Rasmussen LB, Hollenbach B, Laurberg P, Carlé A, Hög A, Jørgensen T, Vejbjerg P, Ovesen L, Schomburg L.
J Trace Elem Med Biol. 2009;23(4):265-71. doi: 10.1016/j.jtemb.2009.03.009.

Down-regulation of the hepatic selenoprotein biosynthesis machinery impairs selenium metabolism during the acute phase response in mice.
Renko K, Hofmann PJ, Stoedter M, Hollenbach B, Behrends T, Köhrle J, Schweizer U, Schomburg L.
FASEB J. 2009 Jun;23(6):1758-65. doi: 10.1096/fj.08-119370.

Selenium supplementation fails to correct the selenoprotein synthesis defect in subjects with SBP2 gene mutations.
Schomburg L, Dumitrescu AM, Liao XH, Bin-Abbas B, Hoeflich J, Köhrle J, Refetoff S.
Thyroid. 2009 Mar;19(3):277-81. doi: 10.1089/thy.2008.0397.

2008

Hepatic selenoprotein P (SePP) expression restores selenium transport and prevents infertility and motor-incoordination in Sepp-knockout mice.
Renko K, Werner M, Renner-Müller I, Cooper TG, Yeung CH, Hollenbach B, Scharpf M, Köhrle J, Schomburg L, Schweizer U.
Biochem J. 2008 Feb 1;409(3):741-9.

New assay for the measurement of selenoprotein P as a sepsis biomarker from serum.
Hollenbach B, Morgenthaler NG, Struck J, Alonso C, Bergmann A, Köhrle J, Schomburg L.
J Trace Elem Med Biol. 2008;22(1):24-32. doi: 10.1016/j.jtemb.2007.11.003.